Objective: Ghrelin gene polymorphism has some association with coronary artery diseases (CAD). We conducted this study to examine the possible association between the polymorphism of Ghrelin gene (Arg51Gln) and CAD. Design and methods: This case-control study was undertaken with patients who were referred to referral heart center, in 2011 in Mazandaran province. DNA extractions were performed, and the Ghrelin region was amplified using PCR. The presence of the Arg51Gln polymorphism of Ghrelin was determined using the restriction fragment length polymorphism ( RFLP ) method. Results: Mean serum level of Ghrelin in the CAD group was significantly higher than in the control group (p<0.0001). We did not find any SNP (with AA genotype) in Ghrelin gene and found six polymorphisms (with GA genotype) in the Ghrelin gene. In CAD patients, smoking, creatinine and triglyceride levels were significantly higher than in the control group (P=0.011, P=0.001and P=0.021) respectively. There was not a significant relationship between genotype distributions and serum levels of the Ghrelin gene (P=0.86). Conclusion: This study indicates that mean serum level of Ghrelin in the CAD group was significantly higher than in the control group and in CAD patients, smoking, creatinine and triglyceride levels were significantly higher than in the control group.

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