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Ponte Academic Journal
Feb 2020, Volume 76, Issue 2

PHYSICOCHEMICAL AND BIOLOGICAL CHARACTERIZATION OF ESSENTIAL OILS EXTRACTED FROM A MEDICINAL PLANT (MENTHA AQUATICA) IN THE REGION OF EL OUED

Author(s): MAHBOUB Nasma ,SLIMANI Noureddine, BENINE Romaissa, REDOUANI Kaouthar, KHELIL Aminata

J. Ponte - Feb 2020 - Volume 76 - Issue 2
doi: 10.21506/j.ponte.2020.2.4



Abstract:
The aim of this work is the extraction of essential oils (EOs) from Mentha aquatica by using the hydrodistillation technique and to study their biological activities including antimicrobial activity and antioxidant activity. The extraction yield obtained is 5.136%. These essential oils have a liquid aspect, white color and aromatic odor. Concerning the physical properties, we found that the pH is equal to 7.55, the refractive index is 1.339, and the miscibility with ethanol estimated at (1v/6.4v of ethanol). So our EOs are basic and soluble. Moreover, chemical analyzes shows that the acid number is 2.204, the ester number is 10.019, the iodine number is 0.158 and the saponification number is 12.21. Furthermore, the study of antioxidant activity using the DPPH method shows that the IC50 value of essential oil of Mentha aquatica. is 0.976 mg / ml and 0.007mg / ml for ascorbic acid . In order to know the efficiency of these essential oils on some pathogenic bacterial strains such as Escherichia coli ATCC 25922 and Bacillus subtilis ATCC 6633, we were found that they had a weak and limited activity against the bacteria studied.
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