This work aims to study the pollen grains spectrum (quantitative and qualitative analysis) and the physicochemical parameters of 14 selected honeys collected from the north-eastern of Algeria and, one foreign sample from Tunisia. Also, an inventory of major important melliferous plants of Souk-Ahras Province was carried out to survey and to valorise the melliferous flora. The results showed that the pollen richness varies between 0 and 499600 pollen grains per 1g of honey, belonging to 36 botanical families with 65 genera and 45 species were identified in the studied honey samples. According to Maurizio’s classes (1939), 33% of samples belong to Class V, 7% to Class IV and Class III, Class II with 13%, and 40% in Class I. For the realized inventory, a total of 108 melliferous plant species distributed in 40 families were recorded. Asteraceae, Rosaceae and, Fabaceae were the most represented families. The most used flowers were white, yellow, and, purple. Pollen and nectar together were the most food collected by the bees (64%). For the physicochemical proprieties, the quality of the studied honeys was included in international standards except for the HMF content in samples M8 and M11.

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