Previously was shown that Infrasound (IS) at 4 Hz (30dB) frequency had modulating effect on heart muscle contractility. The molecular mechanism underlying this influence is not clear yet. The purpose of the present work is to study the molecular mechanism of infrasound. We have studied the effect of 4Hz (30dB) infrasound on heart muscle contractility, and Na/K pomp activity. We also measured the intracellular concentration of Ca2+ ions by 45Ca, and the content of intracellular cyclic nucleotides (cAMP, cGMP). Methods: Removed snail (Helix pomatia) hearts were cannulated and suspended in bath with physiological solution (PS). PS was exposed to 4Hz (30dB) IS for 30 minutes. 45Ca uptake by muscles incubated in normal and IS-treated PS was measured by 45Ca isotope. 45Ca-uptake and intracellular cyclic nucleotides contents (cAMP and cGMP) were measured by Wallac 1450 liquid scintillation counter. Results: IS at 4 Hz (30dB) frequency increased the amplitude and frequency of heart muscle contractility as well as the intracellular Ca2+ ions concentration. By measuring the content of intracellular cyclic nucleotides (cAMP, cGMP) we observed that by the increase of intracellular Ca2+ concentration caused the increase of cGMP content and decrease of cAMP contents in the cells.

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