Today, mtDNA typing is utilized primarily in cases in which the nuclear DNA is too degraded or cannot be recovered in sufficient quantities to be typed. Laboratory of Genetic of Royal Gendarmerie in Morocco is actively involved in using mtDNA for forensic identifications of human skeletal remains. The mitochondrial DNA analysis method was established in laboratory for identification of human skeletal remains by typing bone samples obtained from four forensic cases of unidentified skeletal remains. Mitochondrial DNA sequences of two hypervariable regions HVI and HVII in the control region were determined. Reproducible results were obtained for bones and teeth up to 52 years old. Bone and teeth samples were pulverized to fine powder, decalcified and DNA was extracted. A 341-bp fragment from HVI and a 267-bp fragment from HVII of the mtDNA control region were amplified. After sequencing of the PCR products, mitotypes were compared to the revised Cambridge Reference Sequence (rCRS) and a phylogenetic tree was built.

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