Within the framework of the search for producing thermophilics micro-organisms of xylanases thermostable used in the industry of the paper mill and another can be used in dye industry, nutrition (diabetes) and in the exploration of intestinal permeability (changed to Dxylose, absorbed by the intestine after ingestion, not metabolized, excreted in the urine). The sifting of the xylanolytic activity of some Actinomycetes thermophilics made it possible to select a stock potentially more active and thermostable. Thus, starting from the chicken compost (Annaba, Algeria), three thermophilics stocks of Actinomycetes are insulated and identified by molecular techniques (PCR) while being based on gene ARNr 16S and sequencing of the totality of the fragment (1511 bp) with alignment with the BLAST confirming their membership of the Actinomadura kind. The isolated stocks autochtones are new species from where interest to develop them. Among these three stocks, the secret species Actinomadura keratinilytica Cpt 20 a very active and thermostable xylanase. In addition part of the gene xylanase (probe xylanase) of size 320 bp is isolated and sequenced, which made it possible to genetically attach this enzyme to the family of the beta (1-4) endoxylanases. The totality of gene is isolated in two fragments from sizes respectively 700 bp and 500 bp (cloning) are in the course of work for if required gathering the two fragments (assembly PCR) and characterizing the totality of gene.

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