Ponte Academic Journal Sep 2019, Volume 75, Issue 9 |
POTENTIAL PROBIOTIC EVALUATION ON IN VITRO OF LEUCONOSTOC MESENTEROIDES ISOLATED FROM ALGERIAN RAW GOAT MILK Author(s): Hansal Nabila ,Benmchernene Zineb, Kihal Mebrouk J. Ponte - Sep 2019 - Volume 75 - Issue 9 doi: 10.21506/j.ponte.2019.9.7 Abstract: This paper investigates the probiotic potential of 4 strains of Leuconostoc mesenteroides (W9, W10, LY36 and LN31) identified phenotypically and with the MALDI-TOF technique. The strains were isolated from raw goat�s milk samples collected in the north western region of Algeria. The effects of probiotic tests on the strains demonstrated that strains indicate resistance to 33, 33% of the 15 antibiotics used, while none of the isolates showed hemolytic activity. Similarly, they were able to survive under conditions of acid, bile salt, pepsin and the stomach-stimulus test for 4 h at 37 �C. Whereas for the percentage of hydrophobicity the highest value was (94.68%) for the LN31 strain whereas the low value was (78.92%) for the W9 strain. Auto-aggregation time of these four Leuconostoc mesenteroides strains was between 15 minutes with W9 and 75 minutes with LY36. While the co-aggregation of the strains tested for Leuconostoc mesenteroides with the Salmonella thyphi pathogen strain ranged between (47.55% for W10) and (68.94% for LN31). However, all strains tested have adherence to epithelial cells of the chicken colon with varying degrees of each other. These results suggest that strains isolate from Algerian raw goat�s milk have very good potential to be used as probiotics.
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