Background: Staphylococcal infections are reported to cause very important problems in hospitalized and immunocompressed patients in worldwide. Biofilm producing bacteria that resist to immune defence, antibiotics and many antimicrobial agents cause chronic and recurrent infections such as indwelling device-associated infections. Staphylococcus epidermidis and Staphylococcus aureus that are mostly known pathogenic strains can produce biofilm that causes bacterial attachment and colonization to prosthetic surfaces and bacterial spread to whole body. Method: This study was performed to correlate biofilm production with methicillin resistance of Staphylococcus aureus and Staphylococcus epidermidis. ?2 test was used to detect existence of significance between the groups. Results: In this study, 75% of MRSA (21/28, methicillin resistant Staphylococcus aureus), 74% of MSSA (39/53, methicillin sensitive Staphylococcus aureus), 80% of MRSE (56/70, methicillin resistant Staphylococcus epidermidis) and 64% of MSSE (138/217, methicillin sensitive Staphylococcus epidermidis) strains were detected as biofilm producers by tube method (TM), congo red agar method (CRA) and micro titer plate method (MTP) as gold standart method, and oxacillin and cefoxitin susceptibilities of strains were detected by Kirby Bauer disc diffusion method. Conclusion: This study concluded that the rate of methicillin resistance of biofilm producer Staphylococcus strains are higher than non-biofilm producer Staphylococcus strains, and MRSE strains were more related with biofilm production (80%, 56/70) than MSSE strains (64%, 138/217) significantly. Keywords: Biofilm Production, Antimicrobial Resistance, Methicillin, Staphylococcus aureus, Staphylococcus epidermidis.

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