Aims: We evaluated the effects of different doses of colchicine, atorvastatin, and their combinations on oxidative stress, apoptosis, and autophagic activities in an atherosclerotic model with lipopolysaccharide-induced inflammation in endothelial-macrophage co-culture cells. Methods: HUVEC and macrophage cells were used in study. HUVEC–macrophage co-culture was treated with 10 ng/ml lipopolysaccharides for 24 h to establish the inflammatory effect observed in the atherosclerotic process. LPS-treated cells were incubated with the concentrations of atorvastatin, colchicine and atorvastatin–colchicine combination. Apoptosis, autophagy, total antioxidant status, and total oxidant status was examined from cell lysates Results: When TAS and TOS values of the control, LPS, and drug groups measured at 24 and 48 h, a significant difference was observed between the control, LPS, and drug groups (P values 0.014 and 0.013, respectively) . A significant difference was found when autophagy and apoptosis levels in the control, LPS, and drug groups measured at 24 and 48 h were evaluated between the groups (P = 0.015 and P= 0.013) Conclusions: Apoptotic activity was found to increase in a dose-dependent manner with both drugs, while autophagic activity was the lowest when atorvastatin was administered alone; colchicine treatment was found to decrease autophagic activity with increase in dose.

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