Background: In order to minimize allergic reactions caused by excessive MMA monomer, hypoallergenic prosthesis base materials that contains diurethan dimethacrilate, polyurethane, polyethylentereftalat and polybuthilentereftalat which have different poymerization alternatives were developed as an alternative to methyl methacrylate (MMA). The aim of this study was to evaluate adhesion of oral streptococci on materials not including MMA, and to examine the influence of surface roughness and pellicle coating on bacterial adhesion. Materials and Methods: 3 different hypoallergenic denture base materials, 1 microwave polymerized poly(methyl methacrylate) (PMMA) and 1 heat polymerized PMMA (QC 20) were assessed. To prepare test specimens (10 x 2 mm), half of the wax patterns were invested in the flask directly in plaster (n=49), while the other half of the patterns were invested against the glass (n=49). For each technique (n=5), the surface roughness of specimens was determined by using a profilometre. Thereafter, the remaining 44 specimens were divided into 2 groups as uncoated and pellicle coated. Adhesion of S. mutans (n=11) and S. sanguinis (n=11) on specimens was assessed by counting bacteria in the images taken by fluorescent microscopy and data were analyzed. SEM images were also used to support study findings. Results: Significant differences were found between materials in terms of surface roughness and the number of adherent bacterial cells (p<0.05). Generally, QC 20 showed the lowest amount of bacterial adhesion in all groups. Pellicle coating reduced the number of adherent bacteria on all resin surfaces (p<0.05). Conclusions: In general, Puran HC, Alldent Sinomer, Polyan and Acron MC showed higher tendency to microbial adhesion when compared to QC 20. Key words: Bacterial adhesion; Hypoallergenic base materials; Surface roughness; Fluorescence microscopy.

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