Mineralization of farmyard manure (FYM) amended soil was assessed in vitro laboratory incubation experiment following wheat cultivation. In the field, treatment were FYM, urea, 50%FYM+50%urea (to provide 120 kg N ha-1), control and wheat varieties (Siran-2010, Atta-habib, Janbaz-2009, and Pirsabak-2008). After wheat harvesting, 100g of fresh soil (sampled from 0-20cm depth) were incubated at 15 and 25�C for five weeks. Janbaz-09 applied with urea+FYM had maximum yield and yield components over other treatments. The mineral N was 30-35 times greater in plots having FYM or FYM+urea over control. At 25 �C, mineral N was higher than at 15 �C. Soil total N was in the order of FYM>FYM+urea>urea>control. SOC decrease over incubation period. The decrease was steeper in FYM and/or urea+FYM than control. At 25 �C, the final SOC content in FYM+urea was 7% greater than at 15 �C over the initial SOC. Likewise, in case of FYM the SOC decreased by 30 % (at 15 �C) and 35% (at 25 �C) from initial SOC. The higher mineral N availability is strongly associated with decreased in SOC. In conclusion, the FYM+urea had increased the wheat production, whereas sole FYM amended soil increased the soil mineral N at 25 �C.

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