According to the climate data during last decades, investigating the comfort zone in different regions and achieving it can greatly help various industries. There are many different methods, so that each one defines the conditions in a certain way and has positive points and small errors depending on the time of presentation. However, nowadays, studies on Human-Bioklima is the basis of urban, development, housing, architecture, tourism planning, and so on. The aim of present study was to identify biological methods and investigating the climate software under study (Kashmar), the lack of complete recognition of comfort conditions and the inappropriate design of different spaces is the main reason for conducting this research. Comfort conditions must be investigated for better coordination of each space with its surroundings and designing each space to enjoy the necessary comfort when using space with the minimum use of heating, cooling, ventilation and others. The present study has investigated some bioclimatic methods in a way that the meteorological data of the region is studied in accordance with each of the biochemical methods and, the region\'s climate was investigated through the help of Autodesk Ecotect Analysis software. Finally, all of these studies have been compared in different ways.

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